AUC and orthogonal methods for accurate AAV size distribution analysis

Susumu Uchiyama, Yuki Yamaguchi · Monday, 24 August 2026, 11:15 – 13:15 · Session 2
Room 101172

This workshop will focus on size distribution analysis of AAV samples and cover the following topics:

  • The relationship between sample components and their sizes
  • The relationship between size distributions and different analytical methods, including the principles of each method
  • Parameters required for data analysis using each method
  • Size distributions of particles approximately 100 nm or smaller sizes
  • Three types of analytical ultracentrifugation methods
  • Required parameters for accurate quantitation of each species
  • The advantages and limitations of analytical ultracentrifugation
  • Correlations with other analytical methods, Mass Photometry, Charge Detection Mass Spectrometry, Chromatography, ELISA/PCR, and UV absorption spectroscopy