Borries Demeler · Monday, 24 August 2026, 16:45 – 18:45 · Session 4
Room 101125
In this workshop we will explore the challenges of lipid nanoparticle (LNP) characterization and demonstrate how AUC and UltraScan can be used to uniquely address the task of measuring cargo load, hydrodynamic radius, molar mass, and density distributions for LNPs. We will focus on the application of custom grids to tackle the problem of variable partial specific volume (PSV), a result of the density differences between lipids and mRNA cargo. Using D2O density matching, we will add information to fit a third variable, PSV, to the information that can be gathered by AUC in addition to the sedimentation and diffusion coefficients. In this workshop, we will teach how to construct appropriate custom grids, design and analyze density matching experiments, and to produce molar mass, density, and size distribution, and ultimately determine cargo loading. We will use published data to illustrate the methodology.